The lumen geometry and chemistry of a protein nanopore govern its ion-conduction properties. Rather than redesigning an entire pore assembly, or mutating a naturally occurring pore, we built on the bacterial CsgG nanopore scaffold with an integral de novo component, which yielded semisynthetic conducting pores with programmed architecture and distinct ion-conduction profiles, including rectifying behavior.
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References
Van der Verren, S. E. et al. A dual-constriction biological nanopore resolves homonucleotide sequences with high fidelity. Nat. Biotechnol. 38, 1415–1420 (2020). This paper established the dual-constriction CsgG:CsgF architecture on which the present work builds.
Schnaider, ...