In glowing plants, monitoring the ratio of two luminescence colours, rather than luminescence intensity, enabled quantitative plant physiology measurements despite interference of metabolism in light emission. This two-colour approach bypasses metabolic ‘noise’ to provide a consistent, accurate reading of plant immune and hormonal responses, as validated with both fungal and bacterial bioluminescence systems.
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References
Krichevsky, A., Meyers, B., Vainstein, A., Maliga, P. & Citovsky, V. Autoluminescent plants. PLoS ONE 5, e15461 (2010). This study demonstrated autonomously luminescent plants by expressing the bacterial lux pathway in chloroplasts.
Mitiouchkina, T. et al. Plants with genetically encoded autoluminescence. N...